علوم زیستی دریا
Amin Oujifard; Ahmad Shadi; Elaheh Fateh; Seyed Javad Hosseini
Abstract
Sillaginidae family fish from Perciforms, are appropriate candidates for shallow water and coastal aquaculture. At least three species of this family represents in the Persian Gulf. Genetic analysis of Sand whiting Sillago sihama , the most common species of family performed using COI gene. During the ...
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Sillaginidae family fish from Perciforms, are appropriate candidates for shallow water and coastal aquaculture. At least three species of this family represents in the Persian Gulf. Genetic analysis of Sand whiting Sillago sihama , the most common species of family performed using COI gene. During the present study 10 samples were collected from Bushehr and Hormozgan provinces coastal waters. DNA extracted by modified CTAB method. Polymerase chain reactions were performed using universal primers - FISHF1 and FISHR1-. Sequencing results showed a 627 bp amplified fragment. Performing BLAST supported high identity to Sillago sihama species, hence morphometric identification confirms molecular barcoding. Genetic distance of 0.02 was calculated between samples of two areas based on Kimura 2- parameter using Mega software. Constructed phylogenetic tree using neighbor joining method whereas the Acanthopagrus latus sequences was used as an outgroup revealed no differentiation between two stations samples. In conclusion based on the results of the present study, the gene flow was high among studied samples and no significant differentiation was observed between Bushehr and Hormozgan samples. In conclusion no discrete populations differentiated based on the results of the present study.
علوم زیستی دریا
Ali Arshadi; Vahid Yavari; Amin Oujifard; Seyed Mohammad Mousavi
Abstract
In this study, the effect of different dietary nucleotide levels (0, 0.2, 0.4 and 0.6%) was investigated on growth indices and some haemolymph biochemical parameters of Litopenaeus vannamei female broodstock during 30 days.hemolymph was sampled in three times, at the first day of the trial, 21 days (before ...
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In this study, the effect of different dietary nucleotide levels (0, 0.2, 0.4 and 0.6%) was investigated on growth indices and some haemolymph biochemical parameters of Litopenaeus vannamei female broodstock during 30 days.hemolymph was sampled in three times, at the first day of the trial, 21 days (before eyestalk ablation) and 30 days (9 days after eyestalk ablation) after starting the feeding trial. In experimental period, water parameters including temperature, dissolve oxygen, salinity and pH recorded daily as 27.79 ± 1.24 ˚C, 5.78 ± 0.41 ppm, 31.82 ± 3.1 ppt and 7.9 ± 0.18, respectively. haemolymph biochemical parameters of female broodstock including glucose, total cholesterol, triglyceride, calcium, albumin, total soluble proteins, high density lipoprotein and low density lipoprotein measured in three phases (start of feeding, 21 [pre eyestalk ablation period] and 30 days after start feeding) by biochemical standard methods. the results showed that body weight increase, specific growth rate and daily growth rate in samples treated by dietary nucleotide showed no significant difference (p > 0.05) compared to control group. analysis of biochemical heamolymph showed that the glucose, total cholesterol, triglyceride, albumin, total protein were significant increasing (p < 0.05), in all groups compare to control group. In conclusion 0.4 % nucleotide can improve the dietary sexual maturation of Litopenaeus vannamei.
Amin Oujifard; Abdolmohammad Abedian Kenari; Ali Taheri
Volume 12, Issue 3 , September 2014, , Pages 4-14
Abstract
The effects of inulin were studied on the growth indices, intestinal morphology as well as fatty acid profile of the Pacific white shrimp, Litopenaeus vannamei with average weight 3.21±0.03 g for 5 weeks. The experiment was carried in triplicate, was conducted in circular PVC tanks of 300 L capacity ...
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The effects of inulin were studied on the growth indices, intestinal morphology as well as fatty acid profile of the Pacific white shrimp, Litopenaeus vannamei with average weight 3.21±0.03 g for 5 weeks. The experiment was carried in triplicate, was conducted in circular PVC tanks of 300 L capacity with 25 shrimp per tank at the Aquaculture Center (Delvar, Bushehr province). Inulin was added to the diets at a 2 levels (0 and 2 percent). The shrimp were fed the experimental diets to visual satiety five times a day at 08:00, 12:00, 16:00, 20:00 and 24:00 h. No significant differences in growth performances (such as survival, feed conversion ratio (FCR), specific growth rate (SGR), Protein efficiency ratio (PER)) was observed, however, Dietary inulin brought about significantly improved 20:3n3 fatty acid content between the two treatments (P>0.05). Intestine epithelium cells height in the abdominal sections significantly (P< 0.05) were higher in shrimp fed by dietary inulin. The results of this study showed that supplement of 2% of inulin has positive effects on fatty acid profile and intestinal morphology in Litopenaeus vannamei.